show Abstracthide AbstractCercospora leaf blight and purple seed stain are caused by Cercospora kikuchii and other Cercospora species. The fungus can infect leaves and seeds on soybean, and it is controlled by fungicide application. The intensive use of fungicides on soybean in Brazil to control other diseases such as Asian soybean rust has exerted resistance selection pressure on all fungi that occur in the crop. In this study analyzed the main point mutations linked to resistance in the target genes cytb and betatubulin using Illumina sequencing in fifty-six Cercospora kikuchii isolates.